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Sabtu, 6 Julai 2013
Antitumor activity of extract of Zingiber aromaticum
Antitumor activity of
extract of Zingiber aromaticum and its bioactive sesquiterpenoid zerumbone.
Health Sciences and Nutrition, PO Box 10041, Kintore Avenue, Adelaide DC, South
Australia 5000. email@example.com
The anticancer properties of zerumbone (2,6,9
humulatriene-8-one, a sesquiterpenoid) from Zingiber aromaticum were compared
with those of curcumin from Curcuma longa in an in vitro MTT tetrazolium salt
assay using HT-29, CaCo-2, and MCF-7 cancer cells and in an azoxymethane (AOM)-induced
animal model of colon cancer using
aberrant crypt foci (ACFs) as a preneoplastic marker. The IC50 of zerumbone was
approximately 10 mM and that of curcumin was 25 mM. Cell cycle arrest in HT-29
cells was observed at G0/G1 for 10 and 12.5 mM and G2/M for 25 mM after 24 h at
concentrations of 10-25 mM of zerumbone, and a concentration-dependent increase
in apoptosis (2-6% of viable cells) was observed after 48 h using the same
concentration range. Male Sprague-Dawley rats were fed extracts in an AIN diet
prepared from the equivalent of 4% by weight of dried rhizomes of Z. aromaticum
and C. longa. ACFs were induced by two doses (15 mg/kg body weight)
subcutaneously of AOM 1 wk apart, the rats were killed 10 wk later, and the
ACFs were assessed in the colon. Total ACFs were significantly reduced by Z.
aromaticum extract (down 21%, P < 0.05) relative to control, the effect
being most evident with large ACFs (>3 aberrant crypts per focus). Similar
reductions were observed with 4% C. longa extract in the diet (down 24%, P <
0.01) and with 2,000 ppm curcumin, the effect being particularly evident with
large ACFs. The concentration of zerumbone in the Z. aromaticum extract diet
was assayed at 300 ppm and of curcumin in the C. longa extract diet was also 300
ppm, i.e., the extract of C. longa was as effective at one-seventh the
concentration of curcumin as the positive control. Zerumbone is effective as an
anticancer agent, possibly by its apoptosis-inducing and antiproliferative
influences. This latter possibility is currently being investigated.