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Sabtu, 6 Julai 2013
Khasiat Kadok dan Mengkudu Hutan
Piper sarmentosum (Kadok) and Morinda elliptica (Mengkudu).
Research Institute Malaysia (FRIM), Kepong, 52109 Kuala Lumpur.
The antioxidant activity of two edible
medicinal plants commonly used in Malaysian traditional medicine i.e. Piper
sarmentosum (kadok) and Morinda elliptica (mengkudu) were tested for antioxidant activity. The methanolic leave extracts of
kadok and mengkudu, at 250ug/ml, were tested using the Xanthine/Xanthine
Oxidase (X/XOD) Superoxide Scavenging assay. Both extracts showed high superoxide scavenging assay, 88% and 80%
respectively compared to superoxide dismutase (SOD) standard. The crude
extracts were further fractionated using column chromatography and tested for
superoxide scavenging activity, to obtain antioxidant active fractions. Two active fractions were obtained from
kadok, PsFr6-71.3%, PsFr7-71.3%, and
one active fraction from mengkudu, MeFr3-86.6%. These active
fractions were compared against 14 phenolic compound standards. After a series of
HPLC analysis of samples and standards, a natural
antioxidant compound was identified in kadok and mengkudu i.e. Naringenin
(4',5,7-Trihydroxyflavanone) with 75.7% superoxide scavenging activity.
Naringenin is a highly potent natural
antioxidant that has been reported in the raw materials of larch and grapefruit
extracts. Thus, kadok and mengkudu which contain Naringenin, could be used as antioxidant dietary